No, atheists cannot know that God does not exist. They claim they know there is no God — that God does not exist! But how do they know that? Where is the evidence that proves beyond a doubt that there is no God? All they can offer is their own assumption because they refuse to accept God’s counsel.
Atheists admit they do not know everything. It is not possible for humans to know everything there is to know. It is not possible for humans to be everywhere at the same time. Therefore, it is not possible for humans to know there is no God! Since an atheist or group of atheists cannot be everywhere at the same time, then how do they know that God is not at the place where they are not? The very place where they are not, can very well be the place where God is! See how absurd this becomes?
The existence of God is provable by the conclusive evidence that exists all around us. We must examine the evidence and reason to a logical conclusion. But so many turn a blind eye to the evidence. They have their mind made up that they will not believe in God. One cannot be prejudice against the evidence and dismiss it simply because they choose to not to believe it.
The intricate balance of nature proves God exists. Example: Hundreds of animals survive only because certain other animals or plants survive. They are dependent upon one another. Hundreds of plants, flowers, trees, live only by cross pollination made possible by animals. By observing nature, we know God exists! The inspired Word says, “For the invisible things of him from the creation of the world are clearly seen, being understood by the things that are made, even his eternal power and Godhead; so that they are without excuse ” (Romans 1:20).
Atheists cannot prove the origin and existence of a single particle of matter in the universe. They cannot answer the simple question: “Where did matter come from?” Where did the printing press that produced this paper you are now reading come from? Even a child will say, someone made it. If we can understand that someone had to make the paper you are reading, cannot we also understand that God made us and all of creation? No wonder God declares: “The fool hath said in his heart, There is no God. They are corrupt, they have done abominable works…” (Psalm 14:1).